Pseudo Ploidy

DNA ploidy in 2D sections is the aim of the Pseudo Ploidy project.

Overview

Integrated optical density (IOD) is used for quantifying the amount of DNA in nuclei in Feulgen stained monolayers. A histogram of IOD values for each nucleus in a cell population can be analysed to classify a sample into a ploidy category. In 2D sections the IOD approach is not feasible, as we are studying nuclei profiles instead of projections of entire nuclei. We have identified texture features that are area independent and highly correlated with IOD in monolayers. We use these features on segmented nuclei in 2D sections to estimate their DNA content. DNA ploidy in 2D sections has not been properly done yet. Our preliminary results are promising and indicate that we may be able to achieve this.