Micro Tracker

Micro Tracker is a method we established for visualizing results from different analysis methods in a traditional histopathological specimen.

Overview

Tracking and Associating Data

We would like to be able to directly correlate results from methods such as Nucleotyping and DNA ploidy to histopathological classification and grading a comparative cell-by-cell analysis.

Background

The method is developed and implemented into RUST (Research Utility Software Tool, where data is collected and analysed). Histological sections stained with standard Hematoxilin-Eosin (HE) are scanned in the NanoZoomer before they are de-stained and re-stained with Feulgen-Schiff and scanned with high resolution on Z1 (image cytometry system). The individual nuclei are then segmented and analyzed, before each nuclei is retrieved in the original HE-scan aided by Micro Tracker. The result from the nuclei measurements are then classified and defined groups can be chosen and given a clear markation (with different colors, for example) which are drawn around the same nuclei in the original HE-scan. The method also works on parallel sections.

Media